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CD69 Polyclonal antibody, PBS Only (10803-1-PBS)

Filter: AllWBIHCIF-P

WB analysis using 10803-1-AP (same clone as 10803-1-PBS)
human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 10803-1-AP (CD69 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 10803-1-PBS
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human tonsillitis using 10803-1-PBS
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

CD69 Polyclonal antibody, PBS Only

Name: CD69 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10803-1-PBS

CD69 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 10803-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

10803-1-PBS targets CD69 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1094 Product name: Recombinant human CD69 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC007037 Sequence: MSSENCFVAENSSLHPESGQENDATSPHFSTRHEGSFQVPVLCAVMNVVFITILIIALIALSVGQYNCPGQYTFSMPSDSHVSSCSEDWVGYQRKCYFISTVKRSWTSAQNACSEHGATLAVIDSEKDMNFLKRYAGREEHWVGLKKEPGHPWKWSNGKEFNNWFNVTGSDKCVFLKNTEVSSMECEKNLYWICNKPYK Predict reactive species
Full Name	CD69 molecule
Calculated Molecular Weight	23 kDa
Observed Molecular Weight	28 kDa, 32 kDa
GenBank Accession Number	BC007037
Gene Symbol	CD69
Gene ID (NCBI)	969
RRID	AB_2074965
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07108
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.