APC Anti-Mouse CD69 (H1.2F3)

CD69 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

mouse

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

1

CloneNo.

H1.2F3

Cat no : APC-65105

Synonyms

VEA, H1.2F3, CD69 antigen, AIM

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using APC-65105

  1x10^6 untreated or anit-CD3/CD28 treated (3 days) mouse splenocytes were surface stained with 0.5 ug APC Anti-Mouse CD69 (H1.2F3) (APC-65105, Clone: H1.2F3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65105

  1x10^6 anti-CD3/CD28 treated (3 days) mouse splenocytes were surface stained with 0.5 ug APC Anti-Mouse CD69 (H1.2F3) (APC-65105, Clone: H1.2F3)(red), or 0.5 ug APC Armenian Hamster IgG Isotype Control (PIP) (APC-65210, Clone: PIP) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.5 ug APC Anti-Mouse CD69 (H1.2F3) (APC-65105, Clone: H1.2F3) (black, dashed). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

APC-65105 targets CD69 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse dendritic epidermal T cell line Y245
• Full Name: CD69 antigen
• GenBank Accession Number: BC106997
• Gene Symbol: Cd69
• Gene ID (NCBI): 12515
• RRID: AB_2882995
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Publications

Species	Application	Title
mouse	FC	Sci Adv; Nutrient availability regulates the secretion and function of immune cell-derived extracellular vesicles through metabolic rewiring; Authors - Yizhuo Wang