Atlantic Blue™ Anti-Human CD7 (4H9)

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  FC experiment of human PBMCs using AB-65203

  1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD4 and 5 ul Atlantic Blue™ Anti-Human CD7 (AB-65203, Clone:4H9) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using AB-65203

  1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD7 (AB-65203, Clone:4H9) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD7 Monoclonal Antibody for FC

Cat No. AB-65203

Clone No.4H9

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

FC

Synonyms

4H9, CD7 molecule, GP40, LEU-9, T cell antigen CD7

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

AB-65203 targets CD7 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  T-acute lymphoblastic leukemia cells

• Full Name: CD7 molecule
• Calculated Molecular Weight: 240 aa, 25 kDa
• GenBank Accession Number: BC009293
• Gene Symbol: CD7
• Gene ID (NCBI): 924
• ENSEMBL Gene ID: ENSG00000173762
• RRID: AB_2935196
• Conjugate: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 404 nm / 458 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P09564
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).