FC experiment of human PBMCs using Biotin-FcA98204
1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD7 Rabbit RecAb (Biotin-FcA98204, Clone: 242402H8) or Biotin Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and PE-conjugated streptavidin. Cells were then stained with FcZero-rAb™ APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-FcA98042, Clone: 240427E12). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD7 Rabbit RecAb (Biotin-FcA98204, Clone: 242402H8) or Biotin Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and PE-conjugated streptavidin. Cells were then stained with FcZero-rAb™ APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-FcA98042, Clone: 240427E12). Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
Biotin-FcA98204 targets CD7 in FC applications and shows reactivity with human samples.
FC experiment of human PBMCs using Biotin-FcA98204
1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD7 Rabbit RecAb (Biotin-FcA98204, Clone: 242402H8) or Biotin Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and PE-conjugated streptavidin. Cells were then stained with FcZero-rAb™ APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-FcA98042, Clone: 240427E12). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD7 Recombinant antibody
Catalog Number
Biotin-FcA98204
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
