Anti-Mouse CD70 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of Mouse splenic leucocytes using 98212-1-RR (same clone as 98212-1-PBS)

  1x10^6 untreated or LPS treated mouse splenic leukocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98212-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Mouse splenic leucocytes using 98212-1-RR (same clone as 98212-1-PBS)

  1x10^6 LPS treated mouse splenic leukocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 LPS treated Mouse splenic leucocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98212-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98212-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98212-1-RR against Mouse CD70 were performed. The affinity constant is 37.8 pM.

CD70 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98212-1-PBS

Clone No.241553F2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241553F2

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Product Information

98212-1-PBS targets CD70 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD70 fusion protein Eg1294
• Full Name: CD70 antigen
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: NM_011617.2
• Gene Symbol: Cd70
• Gene ID (NCBI): 21948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O55237
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD70, also known as tumor necrosis factor ligand superfamily member 7 (TNFSF7) or CD27 ligand (CD27L), is a transmembrane protein that plays a significant role in the immune system. It is the unique ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) family, and is transiently upregulated upon stimulation of immune cells. CD70 is expressed on lymphocytes and dendritic cells, and its expression can be regulated by activation signals received via toll-like receptors (TLRs), CD40, and the antigen receptor MHC II. It provides complex and not completely understood signaling for B cell development. CD70 is also expressed on various solid tumors and hematolymphoid malignancies, where it may contribute to a poor prognosis. (PMID: 34991665; 26213107; 26098609)