Anti-Mouse CD70 Rabbit Recombinant Antibody

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  FC experiment of Mouse splenic leucocytes using 98212-1-RR

  1x10^6 untreated or LPS treated mouse splenic leukocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Mouse splenic leucocytes using 98212-1-RR

  1x10^6 LPS treated mouse splenic leukocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 LPS treated Mouse splenic leucocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98212-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98212-1-RR against Mouse CD70 were performed. The affinity constant is 37.8 pM.

CD70 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98212-1-RR

Clone No.241553F2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241553F2

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Tested Applications

• Positive FC detected in: LPS treated mouse splenic leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98212-1-RR targets CD70 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD70 fusion protein Eg1294
• Full Name: CD70 antigen
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: NM_011617.2
• Gene Symbol: Cd70
• Gene ID (NCBI): 21948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O55237
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD70, also known as tumor necrosis factor ligand superfamily member 7 (TNFSF7) or CD27 ligand (CD27L), is a transmembrane protein that plays a significant role in the immune system. It is the unique ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) family, and is transiently upregulated upon stimulation of immune cells. CD70 is expressed on lymphocytes and dendritic cells, and its expression can be regulated by activation signals received via toll-like receptors (TLRs), CD40, and the antigen receptor MHC II. It provides complex and not completely understood signaling for B cell development. CD70 is also expressed on various solid tumors and hematolymphoid malignancies, where it may contribute to a poor prognosis. (PMID: 34991665; 26213107; 26098609)