FcZero-rAb® CoraLite® Plus 647 Anti-Human CD70 Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 647 Anti-Human CD70 Rabbit Recombinant Antibody (CL647-FcA98098)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD70 FcZero-rAb^® Recombinant Antibody for FC

Cat No. CL647-FcA98098 Copied!

Clone No. 241325D8

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD27 L, CD27 ligand, CD27L, CD27-L, CD27LG

• 

  FC experiment of U266 using CL647-FcA98098

  1x10^6 U266 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD70 Rabbit RecAb (CL647-FcA98098, Clone: 241325D8) (red) or FcZero-rAb® CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: U266 cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-FcA98098 targets CD70 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD70 molecule
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC000725
• Gene Symbol: CD70
• Gene ID (NCBI): 970
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32970
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD70, also known as tumor necrosis factor ligand superfamily member 7 (TNFSF7) or CD27 ligand (CD27L), is a transmembrane protein that plays a significant role in the immune system. It is the unique ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) family, and is transiently upregulated upon stimulation of immune cells. CD70 is expressed on lymphocytes and dendritic cells, and its expression can be regulated by activation signals received via toll-like receptors (TLRs), CD40, and the antigen receptor MHC II. It provides complex and not completely understood signaling for B cell development. CD70 is also expressed on various solid tumors and hematolymphoid malignancies, where it may contribute to a poor prognosis.