Tested Applications
| Positive WB detected in | PC-3 cells, RAW 264.7 cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65236-1-Ig targets CD71 in WB, IHC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | transferrin receptor (p90, CD71) |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC001188 |
| Gene Symbol | CD71 |
| Gene ID (NCBI) | 7037 |
| ENSEMBL Gene ID | ENSG00000072274 |
| RRID | AB_3085030 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02786 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD71 antibody 65236-1-Ig | Download protocol |
| WB protocol for CD71 antibody 65236-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD71 Monoclonal antibody (Cat# 65236-1-Ig) has 27 citations spanning journals including Cell Death & Differentiation, Phytomedicine, Free Radical Biology and Medicine, Molecular Medicine, Human Reproduction, FASEB Journal, and International Immunopharmacology. Research fields predominantly involve ferroptosis, iron metabolism, and oxidative stress, with applications in neurodegeneration (Parkinson's, TBI), oncology (colorectal, pancreatic, esophageal cancer), reproductive toxicity, cardiovascular injury, pulmonary fibrosis, and inflammatory diseases.
| Species | Application | Title |
|---|---|---|
Cell Death Differ Dysregulation of the TFEB-ATP6V0C axis in microglia exacerbates α-synuclein pathology through impaired lysosomal acidification in Parkinson's disease. | ||
Phytomedicine Liquiritigenin targets transferrin receptor to potentiate ferroptosis sensitivity in colorectal cancer cells. | ||
Mol Med TIMP1 regulates ferroptosis in osteoblasts by inhibiting TFRC ubiquitination: an in vitro and in vivo study | ||
Antioxidants (Basel) Asperosaponin VI Ameliorates Spontaneous Abortion by Inhibiting Trophoblast Ferroptosis via the KEAP1/NRF2/GPX4 Axis. | ||
Free Radic Biol Med Pumilio2 deficiency promotes iron-dependent macrophage inflammation via TfR1 upregulation in lupus. | ||
Free Radic Biol Med BMAL1-mediated circadian-ferroptosis crosstalk drives neuronal vulnerability after TBI. |
