CD71 Monoclonal antibody, PBS Only

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  WB analysis using 65236-1-Ig (same clone as 65236-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 65236-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 65236-1-Ig (same clone as 65236-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 65236-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 65236-1-Ig (same clone as 65236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65236-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 65236-1-Ig (same clone as 65236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65236-1-PBS in a different storage buffer formulation.

CD71 Monoclonal Antibody for WB, IHC

Cat No. 65236-1-PBS

Clone No.H68.4

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, mouse

Applications

Synonyms

H68.4, p90, sTfR, T9, TFR

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65236-1-Ig

Product Information

65236-1-PBS targets CD71 in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• ENSEMBL Gene ID: ENSG00000072274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02786
• Storage Buffer: PBS Only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.