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Anti-Mouse CD71 (RI7217) Rat Recombinant Antibody, PBS Only (65654-1-PBS)

Anti-Mouse CD71 (RI7217) Rat Recombinant Antibody, PBS Only

Name: Anti-Mouse CD71 (RI7217) Rat Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 65654-1-PBS-1MG

CD71 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65654-1-PBS

Clone No. RI7217

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Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

Synonyms

Tfrc, RI7217, TFR, TFR1, TR

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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FC experiment of mouse bone marrow cells using 65654-1-TR (same clone as 65654-1-PBS)
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse TER-119. Cells were not fixed. This data was developed using the same antibody clone with 65654-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse TER-119. Cells were not fixed. This data was developed using the same antibody clone with 65654-1-PBS in a different storage buffer formulation.
FC experiment of mouse bone marrow cells using 65654-1-TR (same clone as 65654-1-PBS)
1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65654-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

65654-1-PBS targets CD71 in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rat / IgG2a
Class	Recombinant
Type	Antibody
Immunogen	N/A Predict reactive species
Full Name	transferrin receptor
GenBank Accession Number	BC054522
Gene Symbol	CD71
Gene ID (NCBI)	22042
RRID	AB_3743278
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q62351
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule, creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.