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Anti-Mouse CD71 Rabbit Recombinant Antibody, PBS Only (98272-1-PBS)

Anti-Mouse CD71 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Mouse CD71 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98272-1-PBS-1MG

CD71 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98272-1-PBS

Clone No. 242122F6

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

Tfrc, 242122F6, TFR, TFR1, TR

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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FC experiment of mouse bone marrow cells using 98272-1-RR (same clone as 98272-1-PBS)
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse TER-119. Cells were not fixed. This data was developed using the same antibody clone with 98272-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse TER-119. Cells were not fixed. This data was developed using the same antibody clone with 98272-1-PBS in a different storage buffer formulation.
FC experiment of mouse bone marrow cells using 98272-1-RR (same clone as 98272-1-PBS)
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6)(red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98272-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6)(red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98272-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98272-1-RR
Biolayer interferometry (BLl) kinetic assays of 98272-1-RR against Mouse CD71 were performed. The affinity constant is 0.88 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

98272-1-PBS targets CD71 in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1064 Product name: Recombinant Mouse Transferrin R protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: N-6His Domain: 89-763 aa of NM_011638.4 Sequence: CKRVEQKEECVKLAETEETDKSETMETEDVPTSSRLYWADLKTLLSEKLNSIEFADTIKQLSQNTYTPREAGSQKDESLAYYIENQFHEFKFSKVWRDEHYVKIQVKSSIGQNMVTIVQSNGNLDPVESPEGYVAFSKPTEVSGKLVHANFGTKKDFEELSYSVNGSLVIVRAGEITFAEKVANAQSFNAIGVLIYMDKNKFPVVEADLALFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPNIPVQTISRAAAEKLFGKMEGSCPARWNIDSSCKLELSQNQNVKLIVKNVLKERRILNIFGVIKGYEEPDRYVVVGAQRDALGAGVAAKSSVGTGLLLKLAQVFSDMISKDGFRPSRSIIFASWTAGDFGAVGATEWLEGYLSSLHLKAFTYINLDKVVLGTSNFKVSASPLLYTLMGKIMQDVKHPVDGKSLYRDSNWISKVEKLSFDNAAYPFLAYSGIPAVSFCFCEDADYPYLGTRLDTYEALTQKVPQLNQMVRTAAEVAGQLIIKLTHDVELNLDYEMYNSKLLSFMKDLNQFKTDIRDMGLSLQWLYSARGDYFRATSRLTTDFHNAEKTNRFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVENLKLRQKNITAFNETLFRNQLALATWTIQGVANALSGDIWNIDNEF Predict reactive species
Full Name	transferrin receptor
Calculated Molecular Weight	86kDa
GenBank Accession Number	NM_011638.4
Gene Symbol	CD71
Gene ID (NCBI)	22042
RRID	AB_3745938
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q62351
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.