FC experiment of mouse bone marrow cells using 98272-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse TER-119. Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse TER-119. Cells were not fixed.
FC experiment of mouse bone marrow cells using 98272-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6)(red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6)(red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Affinity and Kinetic Characterization of 98272-1-RR
Biolayer interferometry (BLl) kinetic assays of 98272-1-RR against Mouse CD71 were performed. The affinity constant is 0.88 nM.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98272-1-RR targets CD71 in FC applications and shows reactivity with mouse samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
FC experiment of mouse bone marrow cells using 98272-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse TER-119. Cells were not fixed.
FC experiment of mouse bone marrow cells using 98272-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 Rabbit Recombinant Antibody (98272-1-RR, Clone: 242122F6)(red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98272-1-RR
Biolayer interferometry (BLl) kinetic assays of 98272-1-RR against Mouse CD71 were performed. The affinity constant is 0.88 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD71 Recombinant antibody
Catalog Number
98272-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
