FcZero-rAb® Biotin Anti-Human CD72 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-FcA98090

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD72 Rabbit RecAb (Biotin-FcA98090, Clone: 241300C1) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with FcZero-rAb™ APC Anti-Human CD19 (4G7) Rabbit IgG Recombinant Antibody (APC-FcA65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-FcA98090

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD72 Rabbit RecAb (Biotin-FcA98090, Clone: 241300C1) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed. Lymphocytes were gated.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD72 FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA98090

Clone No.241300C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

241300C1, B-cell differentiation antigen CD72, CD72 molecule, CD72b, Lyb 2

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98090 targets CD72 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD72 molecule
• Calculated Molecular Weight: 359 aa, 40 kDa
• GenBank Accession Number: BC030227
• Gene Symbol: CD72
• Gene ID (NCBI): 971
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21854
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).