Atlantic Blue™ Anti-Human CD8 (UCHT4)

CD8 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

UCHT4

Cat no : AB-65204

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32



Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

AB-65204 targets CD8 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Thymocytes and Sézary T cells
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for Atlantic Blue™ CD8 antibody AB-65204Download protocol
Standard Protocols
Click here to view our Standard Protocols