APC Anti-Mouse CD80 (B7-1) (16-10A1)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

3

CloneNo.

16-10A1

Cat no : APC-65076

Synonyms

B7 1, B7.1, Cd28l, Cd80, CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1

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Validation Data Gallery

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  FC experiment of Balb/c mouse peritoneal macrophages using APC-65076

  1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.20 ug APC-Anti-Mouse CD80 (B7-1) (APC-65076, clone 16-10A1) (red) or 0.20 ug APC-Armenian Hamster IgG isotype control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

APC-65076 targets CD80 (B7-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHO cell line transfected with mouse B7 (CD80)
• Full Name: CD80 antigen
• GenBank Accession Number: BC131959
• Gene Symbol: Cd80
• Gene ID (NCBI): 12519
• RRID: AB_2882975
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	Small; Tailor-Made Autophagy Cascade Amplification Polymeric Nanoparticles for Enhanced Tumor Immunotherapy; Authors - Xuehua Long
mouse	FC	J Control Release; Self-heating mitochondrion-induced free radical blast for immunogenic cell death stimulation and HCC immunotherapy; Authors - Mengyun Peng
mouse	FC	J Mater Chem B; PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer; Authors - Lian Zhu