CoraLite® Plus 647 Anti-Human CD80 (B7-1) (2D10.4)
CD80 (B7-1) Monoclonal Antibody for FC
Host / Isotype
Mouse / Mouse IgG1, kappa
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-65083
|Positive FC detected in
|Daudi cells, IFN-γ and LPS-stimulated THP-1-derived macrophages
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
|Sample-dependent, check data in validation data gallery
CL647-65083 targets CD80 (B7-1) in FC applications and shows reactivity with Human samples.
|Host / Isotype
|Mouse / Mouse IgG1, kappa
|Calculated molecular weight
|GenBank accession number
|Gene ID (NCBI)
|CoraLite® Plus 647 Fluorescent Dye
|Excitation/Emission maxima wavelengths
|654 nm / 674 nm
|PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
|Store at 2-8°C. Avoid exposure to light.
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)