CoraLite® Plus 647 Anti-Human CD80 (B7-1) (2D10.4)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2D10.4

Cat no : CL647-65083

Synonyms

Activation B7 1 antigen, B7, B7 1, BB1, CD28LG, CD28LG1, CD80, CD80 (B7-1), CD80 molecule, CTLA 4 counter receptor B7.1, LAB7

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Validation Data Gallery

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  FC experiment of Daudi using CL647-65083

  1x10^6 Daudi cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD80 (B7-1) (CL647-65083, Clone: 2D10.4), or CoraLite® Plus 647 Mouse IgG1 Isotype Control. Cells were not fixed.

• 

  FC experiment of Daudi using CL647-65083

  1x10^6 Daudi cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD80 (B7-1) (CL647-65083, Clone: 2D10.4), or CoraLite® Plus 647 Mouse IgG1 Isotype Control. Cells were not fixed.

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  FC experiment of THP-1 using CL647-65083

  THP-1 cells were differentiated into macrophages by incubation in the presence of phorbol 12-myristate 13-acetate (PMA). THP-1-derived macrophages were surface stained with CoraLite® Plus 647 Anti-Human CD80 (B7-1) (CL647-65083, Clone: 2D10.4) (dashed line), or polarized in M1 macrophages by incubation with IFN-γ (20 ng/ml) and LPS (100 ng/ml) for 48h and then surface stained with CoraLite® Plus 647 Anti-Human CD80 (B7-1) (CL647-65083, Clone: 2D10.4) (red) or isotype control (blue, solid line). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Daudi cells, IFN-γ and LPS-stimulated THP-1-derived macrophages

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65083 targets CD80 (B7-1) in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD80 molecule
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC042665
• Gene Symbol: CD80
• Gene ID (NCBI): 941
• RRID: AB_2883670
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)