CoraLite® Plus 488 Anti-Human CD80 Rabbit scFv Antibody

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• CoraLite® Plus 488 Anti-Human CD80 Rabbit scFv Antibody (CL488-SF98384)

• Site-specific conjugation

CD80 Recombinant Antibody for FC

Cat No. CL488-SF98384

Clone No.242913B11

Host / Type

Rabbit / scFv

Reactivity

human

Applications

FC

Synonyms

Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1, B7-1

• 

  FC experiment of Raji using CL488-SF98384

  1x10^6 Raji cells (left) or HUVEC cells (right) were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Human CD80 Rabbit scFv Antibody (CL488-SF98384, Clone: 242913B11) (red) or 0.25 ug CoraLite® Plus 488 Rabbit scFv Isotype Control (CL488-SF98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: Raji cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-SF98384 targets CD80 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  CatNo: Eg3505

  Product name: Recombinant Human CD80/B7-1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 35-242 aa of NM_005191

  Sequence: VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN

• Full Name: CD80 molecule
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: NM_005191
• Gene Symbol: CD80
• Gene ID (NCBI): 941
• RRID: AB_3748013
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Source: Anti-Human CD80 scFv from clone 242913B11, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)