Tested Applications
| Positive FC detected in | mouse peritoneal macrophages |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65076-1-Ig targets CD80 in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CHO cell line transfected with mouse B7 (CD80) Predict reactive species |
| Full Name | CD80 antigen |
| GenBank Accession Number | BC131959 |
| Gene Symbol | CD80 |
| Gene ID (NCBI) | 12519 |
| RRID | AB_2918380 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q00609 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD80 antibody 65076-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD80 (16-10A1), catalog number 65076-1-Ig, has 13 cited publications appearing in journals including Advances in Materials, Journal of Controlled Release, Matter Today Bio, Phytomedicine, International Journal of Biological Macromolecules, Biomolecules, Neoplasia, and others. Research fields span cancer immunotherapy, ferroptosis, nanomedicine, inflammation, autophagy, atherosclerosis, and tissue repair, with most studies utilizing flow cytometry in mouse models.
| Species | Application | Title |
|---|---|---|
Adv Mater Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication. | ||
MedComm (2020) Full-active pharmaceutical ingredient nanosensitizer for augmented photoimmunotherapy by synergistic mitochondria targeting and immunogenic death inducing | ||
J Control Release Unsaturated fatty acid-doped liposomes deliver piperine to deactivate defensive mechanism for ferroptosis in cancer therapy. | ||
Phytomedicine Daphnetin alleviates influenza pneumonia by enhancing chaperone-mediated autophagy of KEAP1 through Heat shock cognate 71 kDa protein. | ||
Adv Healthc Mater A Peritoneum-Inspired Biomimetic Asymmetric Patch for Functional Repair and Anti-Adhesion in Abdominal Wall Defects. | ||
Mater Today Bio Mitochondria-targeted cuproptosis-driven nanoplatform for synergistic photothermal/chemodynamic therapy and systemic antitumor immunotherapy. |
