Anti-Mouse CD80 (B7-1) (16-10A1)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

1

CloneNo.

16-10A1

Cat no : 65076-1-Ig

Synonyms

B7 1, B7.1, Cd28l, Cd80, CD80 (B7-1), CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1

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Validation Data Gallery

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  FC experiment of BALB/C mouse peritoneal macrophages using 65076-1-Ig

  1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.5 ug Anti-Mouse CD80 (B7-1) (65076-1-Ig, Clone:16-10A1) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100 or unstained. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

65076-1-Ig targets CD80 (B7-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHO cell line transfected with mouse B7 (CD80)
• Full Name: CD80 antigen
• GenBank Accession Number: BC131959
• Gene Symbol: Cd80
• Gene ID (NCBI): 12519
• RRID: AB_2918380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide, pH 7.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	Biomolecules; Glucose Transporter Glut1-Dependent Metabolic Reprogramming Regulates Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages; Authors - Alex Cornwell