PE Anti-Mouse CD80 (B7-1) (16-10A1)
CD80 (B7-1) Monoclonal Antibody for FC
Host / Isotype
Armenian Hamster / IgG
Reactivity
Mouse and More (1)
Applications
FC
Conjugate
PE Fluorescent Dye
CloneNo.
16-10A1
Cat no : PE-65076
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | mouse peritoneal macrophages |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Published Applications
FC | See 1 publications below |
Product Information
PE-65076 targets CD80 (B7-1) in FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Cited Reactivity | mouse |
Host / Isotype | Armenian Hamster / IgG |
Class | Monoclonal |
Type | Antibody |
Immunogen | CHO cell line transfected with mouse B7 (CD80) |
Full Name | CD80 antigen |
GenBank accession number | BC131959 |
Gene symbol | Cd80 |
Gene ID (NCBI) | 12519 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
Background Information
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
Protocols
Product Specific Protocols | |
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FC protocol for PE CD80 (B7-1) antibody PE-65076 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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Asian J Pharm Sci Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors |