PE Anti-Mouse CD80 (B7-1) (16-10A1)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

1

CloneNo.

16-10A1

Cat no : PE-65076

Synonyms

B7 1, B7.1, Cd28l, Cd80, CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1

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Validation Data Gallery

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  FC experiment of Balb/c mouse peritoneal macrophages using PE-65076

  1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.20 ug PE-Anti-Mouse CD80 (B7-1) (PE-65076, clone 16-10A1) (red) or 0.20 ug PE-Armenian Hamster IgG isotype control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

• FC: See 1 publications below

Product Information

PE-65076 targets CD80 (B7-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHO cell line transfected with mouse B7 (CD80)
• Full Name: CD80 antigen
• GenBank accession number: BC131959
• Gene symbol: Cd80
• Gene ID (NCBI): 12519
• Conjugate: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	Asian J Pharm Sci; Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors; Authors - Xudong Fan