Anti-Mouse CD80 Rabbit Recombinant Antibody

• 

  FC experiment of mouse peritoneal macrophages using 98158-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using 98158-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98158-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98158-1-RR against Mouse CD80/B7-1 were performed. The affinity constant is 1.05 nM.

CD80 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98158-1-RR

Clone No.241371E12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ly 53, CD80 antigen, Cd28l, B7.1, B7 1

---

Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98158-1-RR targets CD80 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD80 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_009855.2
• Gene Symbol: CD80
• Gene ID (NCBI): 12519
• RRID: AB_3672301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q00609-1
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)