CoraLite® Plus 647 Anti-Mouse CD81 (EAT2)

CD81 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

EAT2

Cat no : CL647-65220

Synonyms

Cd81, CD81 antigen, Tapa 1, Tapa1, Tspan28

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL647-65220

  1X10^6 mouse splenocytes were surface stained with 1 ug CoraLite® Plus 647 Anti-Mouse CD81 (CL647-65220, Clone: EAT2) (red) or 1 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65220 targets CD81 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD81+ mouse B lymphoma 38C13
• Full Name: CD81 antigen
• GenBank Accession Number: BC011433
• Gene Symbol: Cd81
• Gene ID (NCBI): 12520
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).