CD82 Monoclonal antibody

CD82 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E7A4

Cat no : 66803-1-Ig

Synonyms

4F9, C33, C33 antigen, CD82, CD82 antigen, CD82 molecule, GR15, IA4, Inducible membrane protein R2, KAI1, Metastasis suppressor Kangai 1, R2, SAR2, ST6, Tetraspanin 27, Tspan 27, TSPAN27



Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, Raji cells, K-562 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66803-1-Ig targets CD82 in WB, IHC,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD82 fusion protein Ag17513
Full Name CD82 molecule
Calculated molecular weight 30 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000726
Gene symbol CD82
Gene ID (NCBI) 3732
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

Protocols

Product Specific Protocols
WB protocol for CD82 antibody 66803-1-IgDownload protocol
IHC protocol for CD82 antibody 66803-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols