CoraLite® Plus 488-conjugated CD82 Monoclonal antibody

CD82 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E7A4

Cat no : CL488-66803

Synonyms

4F9, C33, C33 antigen, CD82, CD82 antigen, CD82 molecule, GR15, IA4, Inducible membrane protein R2, KAI1, Metastasis suppressor Kangai 1, R2, SAR2, ST6, Tetraspanin 27, Tspan 27, TSPAN27



Tested Applications

Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66803 targets CD82 in IF applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD82 fusion protein Ag17513
Full Name CD82 molecule
Calculated Molecular Weight 30 kDa
GenBank Accession NumberBC000726
Gene Symbol CD82
Gene ID (NCBI) 3732
RRIDAB_2919374
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD82 antibody CL488-66803Download protocol
Standard Protocols
Click here to view our Standard Protocols