CD83 Recombinant monoclonal antibody

• 

  WB analysis using 84796-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84796-3-RR (CD83 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD83 Recombinant Antibody for WB, ELISA

Cat No. 84796-3-RR

Clone No.241855G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD83 molecule, CD83 antigen, BL11, B-cell activation protein, B cell activation protein

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Tested Applications

• Positive WB detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84796-3-RR targets CD83 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CD83 molecule
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_004233.4
• Gene Symbol: CD83
• Gene ID (NCBI): 9308
• ENSEMBL Gene ID: ENSG00000112149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).