Name: APC Anti-Human CD83 (HB15e)
Brand: Proteintech
SKU: APC-65171

Filter: AllFC

FC experiment of Human Monocytes-derived dendritic cells using APC-65171
1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 10 ul APC Anti-Human CD83 (APC-65171, Clone:HB15e) (blue) or APC mouse IgG1 isotype control (black). Cells were not fixed.

This product has been discontinued. We recommend APC-FcA65581 as an alternative.
For more information, please contact us.

Positive FC detected in

Human monocytes-derived dendritic cells

Application	Dilution
Flow Cytometry (FC)	FC : 15 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

APC-65171 targets CD83 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity	Human, Non-human primates
Host / Isotype	Mouse / Mouse IgG1 kappa
Class	Monoclonal
Type	Antibody
Immunogen	Human CD83-transfected Cos cells Predict reactive species
Full Name	CD83 molecule
Calculated Molecular Weight	205 aa, 23 kDa
GenBank Accession Number	BC030830
Gene Symbol	CD83
Gene ID (NCBI)	9308
ENSEMBL Gene ID	ENSG00000112149
RRID	AB_2918931
Conjugate	APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths	650 nm / 660 nm
Form	Liquid
Purification Method	The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT ID	Q01151
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Product Specific Protocols
FC protocol for APC CD83 antibody APC-65171	Download protocol

Standard Protocols
Click here to view our Standard Protocols