FcZero-rAb® Biotin Anti-Human CD83 (HB15e) Rabbit Recombinant Antibody

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  FC experiment of human monocyte-derived mature dendritic cells using Biotin-FcA65581

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Biotin Anti-Human CD83 (HB15e) Rabbit IgG RecAb (Biotin-FcA65581, Clone: HB15e) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and PE-conjugated streptavidin. Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD83 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA65581

Clone No.HB15e

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, non-human primates

Applications

FC, ELISA

Synonyms

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

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Tested Applications

• Positive FC detected in: human monocyte-derived mature dendritic cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA65581 targets CD83 in FC, ELISA applications and shows reactivity with human, monkey, non-human primates samples.

• Tested Reactivity: human, monkey, non-human primates
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human CD83-transfected Cos cells

• Full Name: CD83 molecule
• Calculated Molecular Weight: 205 aa, 23 kDa
• GenBank Accession Number: BC030830
• Gene Symbol: CD83
• Gene ID (NCBI): 9308
• ENSEMBL Gene ID: ENSG00000112149
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01151
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also be expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).