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Anti-Human CD85j / LILRB1 Rabbit Recombinant Antibody, PBS Only (98471-1-PBS)

Anti-Human CD85j / LILRB1 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CD85j / LILRB1 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98471-1-PBS-1MG

CD85j / LILRB1 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98471-1-PBS

Clone No. 250502H2

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

LILRB1, CD85, CD85 antigen-like family member J, CD85j, FLJ37515

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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Filter: AllIHCFC

IHC staining of human tonsillitis using 98471-1-RR (same clone as 98471-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98471-1-RR (CD85j / LILRB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98471-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of human PBMCs using 98471-1-RR (same clone as 98471-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 Rabbit RecAb (98471-1-RR, Clone: 250502H2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98471-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 Rabbit RecAb (98471-1-RR, Clone: 250502H2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98471-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98471-1-RR (same clone as 98471-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 Rabbit RecAb (98471-1-RR, Clone: 250502H2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98471-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 Rabbit RecAb (98471-1-RR, Clone: 250502H2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98471-1-PBS in a different storage buffer formulation.

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Overview

Tested Applications
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Product Information

98471-1-PBS targets CD85j / LILRB1 in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3701 Product name: Recombinant Human LILRB1/CD85j protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 24-458 aa of BC015731 Sequence: GHLPKPTLWAEPGSVITQGSPVTLRCQGGQETQEYRLYREKKTAPWITRIPQELVKKGQFPIPSITWEHAGRYRCYYGSDTAGRSESSDPLELVVTGAYIKPTLSAQPSPVVNSGGNVTLQCDSQVAFDGFILCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPSRRWWYRCYAYDSNSPYEWSLPSDLLELLVLGVSKKPSLSVQPGPIVAPEETLTLQCGSDAGYNRFVLYKDGERDFLQLAGAQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSEWSAPSDPLDILIAGQFYDRVSLSVQPGPTVASGENVTLLCQSQGWMQTFLLTKEGAADDPWRLRSTYQSQKYQAEFPMGPVTSAHAGTYRCYGSQSSKPYLLTHPSDPLELVVSGPSGGPSSPTTGPTSTSGPEDQPLTPTGSDPQSGLGRH Predict reactive species
Full Name	leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Calculated Molecular Weight	71 kDa
GenBank Accession Number	BC015731
Gene Symbol	LILRB1
Gene ID (NCBI)	10859
RRID	AB_3746102
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8NHL6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.