Tested Applications
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83213-1-RR targets CD86 in IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0802 Product name: Recombinant Mouse CD86 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 24-245 aa of NM_019388 Sequence: VSVETQAYFNGTAYLPCPFTKAQNISLSELVVFWQDQQKLVLYEHYLGTEKLDSVNAKYLGRTSFDRNNWTLRLHNVQIKDMGSYDCFIQKKPPTGSIILQQTLTELSVIANFSEPEIKLAQNVTGNSGINLTCTSKQGHPKPKKMYFLITNSTNEYGDNMQISQDNVTELFSISNSLSLSFPDGVWHMTVVCVLETESMKISSKPLNFTQEFPSPQTYWKE Predict reactive species |
| Full Name | CD86 antigen |
| Calculated Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_019388 |
| Gene Symbol | Cd86 |
| Gene ID (NCBI) | 12524 |
| RRID | AB_3670897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42082-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD86 antibody 83213-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD86 Recombinant monoclonal antibody (230476B2), catalog number 83213-1-RR, has 11 citations. These appear in journals including Nature Communications, Journal of Nanobiotechnology, Acta Pharmaceutica Sinica B, Advanced Science, Acta Pharmacologica Sinica, Journal of Orthopaedic Translation, International Journal of Biological Macromolecules, ACS Applied Materials & Interfaces, Microbial Biotechnology, Experimental Dermatology, and Diabetes/Metabolism Research Reviews. Research fields span radiation enteritis, wound healing, lung injury, neuroinflammation, stroke, bone regeneration, breast cancer immunotherapy, endometriosis, photoaging, and atherosclerosis.
| Species | Application | Title |
|---|---|---|
Nat Commun Efficient active hydrogen delivery for drug-free radiation enteritis therapy in mice. | ||
J Nanobiotechnology Spatiotemporal controlled release of YCM/Cu(2)⁺ hydrogel microneedle system synergistically accelerates chronic wound healing in diabetes. | ||
Acta Pharm Sin B Ferrum@albumin assembled nanoclusters inhibit NF-κB signaling pathway for NIR enhanced acute lung injury immunotherapy. | ||
Adv Sci (Weinh) Dual Physiological Barriers Bypassed by a Silk-Based Supramolecular Protein Delivery Platform for Neuroinflammation Mitigation in Alzheimer's Disease. | ||
Acta Pharmacol Sin MMP-9 inhibitor SB-3CT improves neurological outcomes in ischemic stroke mice by modulation of astrocytic lipid metabolism | ||
J Orthop Translat Engineering multifunctional microspheres for sequential regulation of osteoimmune microenvironment and bone remodeling balance to promote regeneration of osteoporotic bone defects. |
