CoraLite® Plus 647 Anti-Mouse CD86 (GL1)

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  FC experiment of mouse splenocytes using CL647-65068

  1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068, Clone: GL1). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-65068

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068, Clone: GL1)(red) or CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068, Clone: GL1) (black, dashed). Cells were not fixed.

CD86 Monoclonal Antibody for FC

Cat No. CL647-65068

Clone No.GL1

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Synonyms

B7, B7 2, B7.2, B70, Cd28l2, Cd86, CD86 antigen, CLS1, ETC 1, Ly 58, Ly58, MB7, MB7 2, TS/A 2

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Tested Applications

• Positive FC detected in: LPS treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65068 targets CD86 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: LPS-activated CBA/Ca mouse spleen B cells
• Full Name: CD86 antigen
• GenBank Accession Number: BC013807
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• RRID: AB_2883663
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P42082
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)