CoraLite® Plus 647 Anti-Mouse CD86 (GL1)

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  FC experiment of mouse splenocytes using CL647-65068

  1X10^6 untreated (dashed line) or LPS treated mouse splenocytes were surface stained with 1 ug CoraLite® Plus 647 Anti-Mouse CD86 (CL647-65068, Clone: GL1) (red), or 1 ug isotype control antibody (blue). Cells were not fixed.

CD86 Monoclonal Antibody for FC

Cat No. CL647-65068

Clone No.GL1

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Synonyms

B7, B7 2, B7.2, B70, Cd28l2, Cd86, CD86 antigen, CLS1, ETC 1, Ly 58, Ly58, MB7, MB7 2, TS/A 2

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Tested Applications

• Positive FC detected in: LPS treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65068 targets CD86 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: LPS-activated CBA/Ca mouse spleen B cells
• Full Name: CD86 antigen
• GenBank Accession Number: BC013807
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• RRID: AB_2883663
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P42082
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)