FITC Plus Anti-Mouse CD86 (GL1)

CD86 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

Publications

11

CloneNo.

GL1

Cat no : FITC-65068

Synonyms

Cd28l2, B7-2, B70, B7.2, B7 2

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Validation Data Gallery

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  FC experiment of mouse splenocytes using FITC-65068

  1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65068

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1)(red) or 0.25 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1) (black, dashed). Cells were not fixed.

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Tested Applications

• Positive FC detected in: LPS treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 8 publications below

Product Information

FITC-65068 targets CD86 in IF, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: LPS-activated CBA/Ca mouse spleen B cells
• Full Name: CD86 antigen
• GenBank Accession Number: BC013807
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• RRID: AB_2883758
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
	FC	Small; [Not Available]; Authors - Baochan Yang
		J Control Release; Programmed antigen capture-harnessed dendritic cells by margination-hitchhiking lung delivery; Authors - Thi My Hue Huynh
mouse	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	Carbohydr Polym; Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii.; Authors - Yuqing Sun
		Mol Neurobiol; HPGD: An Intermediate Player in Microglial Polarization and Multiple Sclerosis Regulated by Nr4a1; Authors - Mengyang Sun
mouse	FC	Front Bioeng Biotechnol; Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response; Authors - Xinlong Xie