FITC Plus Anti-Mouse CD86 (GL1)

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  IF Staining of mouse spleen using FITC-65068

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using FITC Plus CD86 antibody (FITC-65068, Clone: GL1 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of mouse splenocytes using FITC-65068

  1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1). Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-65068

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1) (red) or 0.25 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1) (black, dashed). Cells were not fixed.

CD86 Monoclonal Antibody for IF-P, FC

Cat No. FITC-65068

Clone No.GL1

Publications(18)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF-P, FC

Synonyms

B7, B7 2, B7.2, B70, B7-2

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Tested Applications

• Positive IF-P detected in: mouse spleen tissue
• Positive FC detected in: LPS treated mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• FC: See 12 publications below

Product Information

FITC-65068 targets CD86 in IF-P, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  LPS-activated CBA/Ca mouse spleen B cells

• Full Name: CD86 antigen
• GenBank Accession Number: BC013807
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• RRID: AB_2883758
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P42082
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	FC	Small; [Not Available]; Authors - Baochan Yang
		J Control Release; Programmed antigen capture-harnessed dendritic cells by margination-hitchhiking lung delivery; Authors - Thi My Hue Huynh
mouse	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	Carbohydr Polym; Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii.; Authors - Yuqing Sun
		Mol Neurobiol; HPGD: An Intermediate Player in Microglial Polarization and Multiple Sclerosis Regulated by Nr4a1; Authors - Mengyang Sun
mouse	FC	Front Bioeng Biotechnol; Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response; Authors - Xinlong Xie