FcZero-rAb® Biotin Anti-Human CD89 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using Biotin-FcA98105

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD89 Rabbit RecAb (Biotin-FcA98105, Clone: 241426E3) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with FcZero-rAb® CoraLite® Plus 405 Anti-Human CD16 (3G8) Rabbit Recombinant Antibody (CL405-FcA65612, Clone: 3G8). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using Biotin-FcA98105

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD89 Rabbit RecAb (Biotin-FcA98105, Clone: 241426E3) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD89 FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA98105

Clone No.241426E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

FCAR, IgA Fc receptor, R

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98105 targets CD89 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: Fc fragment of IgA, receptor for
• Calculated Molecular Weight: 287 aa, 32 kDa
• GenBank Accession Number: BC027953
• Gene Symbol: CD89
• Gene ID (NCBI): 2204
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24071
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

FcαRI (CD89) is a member of the Fc receptor immunoglobulin superfamily, constitutively expressed on cells of the myeloid lineage like neutrophils, eosinophils, monocytes, macrophages, and platelets (PMID: 28103138). CD89 expression and activation are increased in human blood phagocytes following bacterial infection, together with increased expression and association of FcRγ with CD89 (PMID: 34756952). CD89 binds both subclasses of IgA (IgA1 and IgA2) with moderate affinity, but also binds other molecules such as TfR1, C-reactive protein and bacterial ligands. CD89 represents an effective target molecule for immunotherapy mediated by bispecific antibodies (PMID: 37081893).