CD8a Recombinant antibody

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  WB analysis using 86063-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86063-1-RR (CD8A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CD8a Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86063-1-RR

Clone No.243018F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CD8, CD8a molecule, Leu2, MAL, p32

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells, rat thymus tissue, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86063-1-RR targets CD8a in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD8a fusion protein Eg0819
• Full Name: CD8a molecule
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_001145873.1
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• ENSEMBL Gene ID: ENSG00000153563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01732-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.