CoraLite®488 Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

53-6.7

Cat no : CL488-65069

Synonyms

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2



Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

CL488-65069 targets CD8a in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Spleen cells or thymocyte membranes
Full Name CD8 antigen, alpha chain
GenBank accession numberBC030679
Gene symbol Cd8a
Gene ID (NCBI) 12525
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
IF protocol for CL488 CD8a antibody CL488-65069Download protocol
FC protocol for CL488 CD8a antibody CL488-65069Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li
IF

Front Chem

Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency

Authors - Yinghui Shang