Anti-Mouse CD8a Rat scFv Antibody

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• Anti-Mouse CD8a Rat scFv Antibody (SF65666-1-TR)

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  SDS-PAGE of SF65666-1-TR

  Purity of Recombinant ScFv antibody(Cat No.:SF65666-1-TR) was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and condition and stained using Coomassie blue.

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  FC experiment of mouse splenocytes using SF65666-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8a Rat scFv Antibody (SF65666-1-TR, Clone: 53-6.7) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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  FC experiment of mouse splenocytes using SF65666-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8a Rat scFv Antibody (SF65666-1-TR, Clone: 53-6.7) (red) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were not fixed.

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  Affinity and Kinetic Characterization of SF65666-1-TR

  Biolayer interferometry (BLl) kinetic assays of SF65666-1-TR against Mouse CD8a were performed. The affinity constant is 1.41 nM.

CD8a Recombinant Antibody for FC

Cat No. SF65666-1-TR

Clone No.53-6.7

Host / Type

Rat / scFv

Reactivity

mouse

Applications

FC

Synonyms

CD8, 53-6.7, CD8 antigen, alpha chain, CD8α, Ly 2

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

SF65666-1-TR targets CD8a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Type: Rat / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  N/A

• Full Name: CD8 antigen, alpha chain
• GenBank Accession Number: BC030679
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• RRID: AB_3750142
• Conjugate: Unconjugated
• Source: Anti-Mouse CD8a scFv from clone 53-6.7, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).