Biotin Anti-Human CD8b Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using Biotin-98103

  1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human CD8b Rabbit RecAb (Biotin-98103, Clone:241326A7) and PE-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (APC-65570, Clone: UCHT1). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98103

  1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human CD8b Rabbit RecAb (Biotin-98103, Clone: 241326A7) and PE-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

CD8b Uni-rAb^® Recombinant Antibody for FC

Cat No. Biotin-98103

Clone No.241326A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD8, 241326A7, CD8b molecule, CD8B1, Leu2

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98103 targets CD8b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD8b molecule
• Calculated Molecular Weight: 243 aa, 27 kDa
• GenBank Accession Number: BC100913
• Gene Symbol: CD8B
• Gene ID (NCBI): 926
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10966
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.