Mouse CD90.1 Recombinant monoclonal antibody, PBS Only

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• Mouse CD90.1 Recombinant monoclonal antibody, PBS Only (84832-1-PBS)

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  WB analysis using 84832-1-RR (same clone as 84832-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84832-1-RR (CD90.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD90.1 antibody (84832-1-RR, Clone: 241542D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84832-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84832-1-RR against Mouse CD90.1 were performed. The affinity constant is 17.0 nM.

CD90.1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84832-1-PBS

Clone No.241542D7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD90, Thy1, Thy 1, 241542D7

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Product Information

84832-1-PBS targets CD90.1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: thymus cell antigen 1, theta
• Observed Molecular Weight: 25 kDa
• Gene Symbol: CD90
• Gene ID (NCBI): 21838
• RRID: AB_3672141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593).