CD90.1 Recombinant antibody

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  WB analysis using 84832-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84832-1-RR (CD90.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84832-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84832-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 84832-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD90.1 antibody (84832-1-RR, Clone: 241542D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84832-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84832-1-RR against Mouse CD90.1 were performed. The affinity constant is 17.0 nM.

CD90.1 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84832-1-RR

Clone No.241542D7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD90, Thy1, Thy 1, 241542D7

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Tested Applications

• Positive WB detected in: FVB mouse brain tissue, FVB mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84832-1-RR targets CD90.1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: thymus cell antigen 1, theta
• Observed Molecular Weight: 25 kDa
• Gene Symbol: CD90
• Gene ID (NCBI): 21838
• RRID: AB_3672141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593).