FcZero-rAb® Biotin Anti-Human CD97 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human CD97 Rabbit Recombinant Antibody (Biotin-FcA98094-2)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD97 FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA98094-2 Copied!

Clone No. 241335D8

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen

• 

  FC experiment of human PBMCs using Biotin-FcA98094-2

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD97 Rabbit RecAb (Biotin-FcA98094-2, Clone: 241335D8) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with FcZero-rAb™ APC Anti-Human CD3 (OKT3) Rabbit Recombinant Antibody (APC-FcA65569, Clone: OKT3). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using Biotin-FcA98094-2

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD97 Rabbit RecAb (Biotin-FcA98094-2, Clone:241335D8) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98094-2 targets CD97 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD97 molecule
• Calculated Molecular Weight: 722 aa, 79 kDa
• GenBank Accession Number: BC026690
• Gene Symbol: CD97
• Gene ID (NCBI): 976
• RRID: AB_3746911
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48960
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).