Biotin Anti-Human CD98/SLC3A2 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-98190

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD98/SLC3A2 Rabbit RecAb (Biotin-98190, Clone: 241755D6) or Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98190

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD98/SLC3A2 Rabbit RecAb (Biotin-98190, Clone: 241755D6) (red) or Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD98/SLC3A2 Uni-rAb^® Recombinant Antibody for FC

Cat No. Biotin-98190

Clone No.241755D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD98, SLC3A2, 241755D6, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98190 targets CD98/SLC3A2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1948

  Product name: Recombinant Human CD98/SLC3A2 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 206-630 aa of NM_002394.5

  Sequence: RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

• Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Calculated Molecular Weight: 68 kDa
• GenBank Accession Number: NM_002394.5
• Gene Symbol: CD98
• Gene ID (NCBI): 6520
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08195-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter, encoded by the SLC7A5) and an 85-kDa glycosylated heavy chain (CD98hc, encoded by the SLC3A2) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).