FITC Plus Anti-Mouse CD98/SLC3A2 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using FITC-98128

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse TER-119, and 0.25 ug FITC Plus Anti-Mouse CD98/SLC3A2 Rabbit RecAb (FITC-98128, Clone:241247F1), or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-98128

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD98/SLC3A2 Rabbit RecAb (FITC-98128, Clone:241247F1) (red), or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were not fixed.

CD98/SLC3A2 Uni-rAb^® Recombinant Antibody for FC

Cat No. FITC-98128

Clone No.241247F1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241247F1, 4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98128 targets CD98/SLC3A2 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1374

  Product name: Recombinant Mouse CD98 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: N-6*His

  Domain: 100-526 aa of NM_008577.4

  Sequence: APRCRELPVQRWWHKGALYRIGDLQAFVGRDAGGIAGLKSHLEYLSTLKVKGLVLGPIHKNQKDEINETDLKQINPTLGSQEDFKDLLQSAKKKSIHIILDLTPNYQGQNAWFLPAQADIVATKMKEALSSWLQDGVDGFQFRDVGKLMNAPLYLAEWQNITKNLSEDRLLIAGTESSDLQQIVNILESTSDLLLTSSYLSNSTFTGERTESLVTRFLNATGSQWCSWSVSQAGLLADFIPDHLLRLYQLLLFTLPGTPVFSYGDELGLQGALPGQPAKAPLMPWNESSIFHIPRPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFHALSSSPDLFSYIRHWDQNERYLVVLNFRDSGRSARLGASNLPAGISLPASAKLLLSTDSARQSREEDTSLKLENLSLNPYEGLLLQFPFVA

• Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: NM_008577.4
• Gene Symbol: CD98/SLC3A2
• Gene ID (NCBI): 17254
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10852-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).