CoraLite® Plus 488-conjugated CD99 Recombinant antibody

CD99 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-84580-4
Clone No.241737H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

12E7, 241737H1, CD99 antigen, CD99 molecule, E2 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84580-4 targets CD99 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein Predict reactive species
Full Name CD99 molecule
Calculated Molecular Weight 19 kDa, 16 kDa, 17 kDa
Observed Molecular Weight28 kDa, 32 kDa
GenBank Accession NumberBC021620
Gene Symbol CD99
Gene ID (NCBI) 4267
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14209
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD99 antibody CL488-84580-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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