CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b


Human, Mouse, rat







Cat no : 67145-1-Ig


CDC25B, CDC25HU2, M phase inducer phosphatase 2

Tested Applications

Positive WB detected inSp2/0 cells, Jurkat cells, U-937 cells
Positive IF detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67145-1-Ig targets CDC25B in WB, IF, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC25B fusion protein Ag27193
Full Name cell division cycle 25 homolog B (S. pombe)
Calculated molecular weight 65 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC006395
Gene symbol CDC25B
Gene ID (NCBI) 994
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.


Product Specific Protocols
WB protocol for CDC25B antibody 67145-1-IgDownload protocol
IF protocol for CDC25B antibody 67145-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols