CDC27; APC3 Polyclonal antibody

CDC27; APC3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10918-1-AP

Synonyms

ANAPC3, APC3, CDC27, CDC27 homolog, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC



Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

10918-1-AP targets CDC27; APC3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC27; APC3 fusion protein Ag1345
Full Name cell division cycle 27 homolog (S. cerevisiae)
Calculated molecular weight 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC011656
Gene symbol CDC27
Gene ID (NCBI) 996
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

Protocols

Product Specific Protocols
WB protocol for CDC27; APC3 antibody 10918-1-APDownload protocol
IHC protocol for CDC27; APC3 antibody 10918-1-APDownload protocol
IF protocol for CDC27; APC3 antibody 10918-1-APDownload protocol
IP protocol for CDC27; APC3 antibody 10918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncotarget

mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27.

Authors - Zhuoying Feng