CDC37L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDC37L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16293-1-AP

Synonyms

CDC37B, CDC37L1, HARC

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Validation Data Gallery

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  WB analysis using 16293-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16293-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16293-1-AP (CDC37L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16293-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16293-1-AP (CDC37L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human kidney tissue, Jurkat cells, LNCaP cells, MCF-7 cells, U2OS cells
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

16293-1-AP targets CDC37L1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC37L1 fusion protein Ag9459
• Full Name: cell division cycle 37 homolog (S. cerevisiae)-like 1
• Calculated Molecular Weight: 337 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC014133
• Gene Symbol: CDC37L1
• Gene ID (NCBI): 55664
• RRID: AB_2244711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IHC	J Cancer; CDC37L1 acts as a suppressor of migration and proliferation in gastric cancer by down-regulating CDK6.; Authors - Li Li ; KD Validated
human	WB,IHC	Cell Death Discov; miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation.; Authors - Li Li