CDC42BPA Polyclonal antibody

CDC42BPA Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31297-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DMPK like alpha, KIAA0451, MRCK, MRCK alpha, MRCKA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHC

WB analysis using 31297-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31297-1-AP (CDC42BPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 31297-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse adrenal gland using 31297-1-AP
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 31297-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat adrenal gland using 31297-1-AP
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 31297-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 31297-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 31297-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	HUVEC cells, K-562 cells, RT-4 cells, SiHa cells
Positive IHC detected in	human ovary cancer tissue, human rectal cancer tissue, mouse adrenal gland tissue, mouse cerebellum tissue, rat adrenal gland tissue, rat cerebellum tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31297-1-AP targets CDC42BPA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag34955 Product name: Recombinant human CDC42BPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 467-556 aa of NM_001366019 Sequence: QTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASE Predict reactive species
Full Name	CDC42 binding protein kinase alpha (DMPK-like)
Calculated Molecular Weight	197 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	NM_001366019
Gene Symbol	CDC42BPA
Gene ID (NCBI)	8476
RRID	AB_3669934
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q5VT25
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc42 binding protein kinase alpha (Cdc42BPA/MRCKα), is a member of the serine/threonine protein kinase family. Previous studies suggested that Cdc42BPA may mediates the Cdc42induced peripheral actin formation to promote cytoskeletal reorganization 5. However, the biological function and clinical significance of Cdc42BPA in colon cancer are unclear. (PMID: 29072916)