CDC42BPA Polyclonal antibody

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  WB analysis using 31297-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31297-1-AP (CDC42BPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CDC42BPA Polyclonal Antibody for WB, ELISA

Cat No. 31297-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DMPK like alpha, KIAA0451, MRCK, MRCK alpha, MRCKA

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Tested Applications

• Positive WB detected in: HUVEC cells, K-562 cells, RT-4 cells, SiHa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31297-1-AP targets CDC42BPA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34955

  Product name: Recombinant human CDC42BPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 467-556 aa of NM_001366019

  Sequence: QTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASE

• Full Name: CDC42 binding protein kinase alpha (DMPK-like)
• Calculated Molecular Weight: 197 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_001366019
• Gene Symbol: CDC42BPA
• Gene ID (NCBI): 8476
• RRID: AB_3669934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5VT25
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdc42 binding protein kinase alpha (Cdc42BPA/MRCKα), is a member of the serine/threonine protein kinase family. Previous studies suggested that Cdc42BPA may mediates the Cdc42induced peripheral actin formation to promote cytoskeletal reorganization 5. However, the biological function and clinical significance of Cdc42BPA in colon cancer are unclear. (PMID: 29072916)