CDC42BPA Polyclonal antibody, PBS Only

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• CDC42BPA Polyclonal antibody, PBS Only (31297-1-PBS)

CDC42BPA Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 31297-1-PBS Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DMPK like alpha, KIAA0451, MRCK, MRCK alpha, MRCKA

• 

  WB analysis using 31297-1-AP (same clone as 31297-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31297-1-AP (CDC42BPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human rectal cancer using 31297-1-AP (same clone as 31297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse adrenal gland using 31297-1-AP (same clone as 31297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 31297-1-AP (same clone as 31297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat adrenal gland using 31297-1-AP (same clone as 31297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat cerebellum using 31297-1-AP (same clone as 31297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 31297-1-AP (same clone as 31297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 31297-1-AP (same clone as 31297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31297-1-AP (CDC42BPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31297-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

31297-1-PBS targets CDC42BPA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34955

  Product name: Recombinant human CDC42BPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 467-556 aa of NM_001366019

  Sequence: QTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASE

• Full Name: CDC42 binding protein kinase alpha (DMPK-like)
• Calculated Molecular Weight: 197 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_001366019
• Gene Symbol: CDC42BPA
• Gene ID (NCBI): 8476
• RRID: AB_3669934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5VT25
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cdc42 binding protein kinase alpha (Cdc42BPA/MRCKα), is a member of the serine/threonine protein kinase family. Previous studies suggested that Cdc42BPA may mediates the Cdc42induced peripheral actin formation to promote cytoskeletal reorganization 5. However, the biological function and clinical significance of Cdc42BPA in colon cancer are unclear. (PMID: 29072916)