CDCA2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86379-1-RR (same clone as 86379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86379-1-RR (CDCA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

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  IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 86379-1-RR (CDCA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

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  IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 86379-1-RR (CDCA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86379-1-RR (same clone as 86379-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDCA2 antibody (86379-1-RR, Clone: 250980D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86379-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86379-1-RR against Human CDCA2 were performed. The affinity constant is below 1 pM.

CDCA2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86379-1-PBS

Clone No.250980D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Cell division cycle-associated protein 2, Repo Man, Repo-Man

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Product Information

86379-1-PBS targets CDCA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11967

  Product name: Recombinant human CDCA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 512-863 aa of BC036214

  Sequence: ATSDEDPNTNIMNINENKNIPKAKNKSESENEPKAGTDSPVSCASITEERVASDSPKPALTLQQGQEFSAGGQNAENLCQFFKISPDLNIKCERKDDFLGAAEGKLQCNRLMPNSQKDCHCLGDVLIENTKESKSQSEDLGRKPMESSSVVSCRDRKDRRRSMCYSDGRSLHLEKNGNHTPSSSVGSSVEISLENSELFKDLSDAIEQTFQRRNSETKVRRSTRLQKDLENEGLVWISLPLPSTSQKAKRRTICTFDSSGFESMSPIKETVSSRQKPQMAPPVSDPENSQGPAAGSSDEPGKRRKSFCISTLANTKATSQFKGYRRRSSLNGKGESSLTALERIEHNGERKQ

• Full Name: cell division cycle associated 2
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC036214
• Gene Symbol: CDCA2
• Gene ID (NCBI): 157313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q69YH5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner.