CDCA3 Polyclonal antibody

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• KD/KO Validated

CDCA3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 15594-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

C8, Cell division cycle-associated protein 3, Gene rich cluster protein C8, Gene-rich cluster protein C8, GRCC8

• 

  IHC staining of human breast cancer using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 15594-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CDCA3 antibody (15594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15594-1-AP targets CDCA3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7951

  Product name: Recombinant human CDCA3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-268 aa of BC002551

  Sequence: MGSAKSVPVTPARPPPHNKHLARVADPRSPSAGILRTPIQVESSPQPGLPAGEQLEGLKHAQDSDPRSPTLGIARTPMKTSSGDPPSPLVKQLSEVFETEDSKSNLPPEPVLPPEAPLSSELDLPLGTQLSVEEQMPPWNQTEFPSKQVFSKEEARQPTETPVASQSSDKPSRDPETPRSSGSMRNRWKPNSSKVLGRSPLTILQDDNSPGTLTLRQGKRPSPLSENVSELKEGAILGTGRLLKTGGRAWEQGQDHDKENQHFPLVES

• Full Name: cell division cycle associated 3
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC002551
• Gene Symbol: CDCA3
• Gene ID (NCBI): 83461
• RRID: AB_2878154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99618
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCA3, also named C8, GRCC8, and TOME1, is an F-box-like protein required for entry into mitosis. It acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at the G2/M phase.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncogene; CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma.; Authors - Xueying Wang
human	IHC	Cancer Cell Int; The inhibitory effect of silencing CDCA3 on migration and proliferation in bladder urothelial carcinoma.; Authors - Dexin Shen ; KD Validated
human	WB,IHC	Naunyn Schmiedebergs Arch Pharmacol; Curcumin targets miR-134-5p to suppress the progression of colorectal cancer through regulating the CDCA3/CDK1 pathway; Authors - Fu Liu
human	WB,IHC,IF,CoIP	Biochem Biophys Res Commun; CDCA3 promotes cell proliferation by activating the NF-κB/cyclin D1 signaling pathway in colorectal cancer.; Authors - Wei Zhang ; KD Validated
human	WB,IHC	Pathol Res Pract; miR-145-5p suppresses proliferation, metastasis and EMT of colorectal cancer by targeting CDCA3.; Authors - Qing Chen
human	WB,IF	J Oncol; CDCA3 Predicts Poor Prognosis and Affects CD8+ T Cell Infiltration in Renal Cell Carcinoma; Authors - Yuanyuan Bai