CDCA4 Polyclonal antibody, PBS Only

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• CDCA4 Polyclonal antibody, PBS Only (11625-1-PBS)

• 

  WB analysis of HeLa using 11625-1-AP (same clone as 11625-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11625-1-AP (same clone as 11625-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11625-1-AP (same clone as 11625-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11625-1-AP (same clone as 11625-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CDCA4 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 11625-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

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Product Information

11625-1-PBS targets CDCA4 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2214

  Product name: Recombinant human CDCA4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-241 aa of BC025263

  Sequence: MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLNRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET

• Full Name: cell division cycle associated 4
• Calculated Molecular Weight: 241 aa, 26 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC025263
• Gene Symbol: CDCA4
• Gene ID (NCBI): 55038
• RRID: AB_2260414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXL8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148].