CDCA5 Monoclonal antibody

CDCA5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2G1D2

Cat no : 67418-1-Ig

Synonyms

CDCA5, p35, SORORIN

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Validation Data Gallery

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  WB analysis using 67418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67418-1-Ig (CDCA5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67418-1-Ig (CDCA5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67418-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDCA5 antibody (67418-1-Ig, Clone: 2G1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

67418-1-Ig targets CDCA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDCA5 fusion protein Ag28958
• Full Name: cell division cycle associated 5
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27~35 kDa
• GenBank Accession Number: BC011000
• Gene Symbol: CDCA5
• Gene ID (NCBI): 113130
• RRID: AB_2882658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human cell division cycle associated 5 (CDCA5), also known as Sororin, is required for stable binding of cohesin to chromatid in the S and G2/M phases and degraded through anaphase-promoting complex-dependent ubiquitination in the G0/G1 phase (PMID: 30808873). Suppression of CDCA5 expression with siRNAs inhibited the growth of lung cancer cells. CDCA5 positivity is an independent prognostic factor for lung cancer patients (PMID: 20551060). The predicted molecular size of CDCA is 27 kd, with an electrophoretic mobility of ~35 kd on SDS-PAGE, possibly due to its isoelectric point (pI) of approximately 10 (PMID: 22580470).

Publications

Species	Application	Title
human	WB	Front Oncol; Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.; Authors - Yingji Chen
human,mouse	WB,IHC,IF	J Transl Med; TPI1 activates the PI3K/AKT/mTOR signaling pathway to induce breast cancer progression by stabilizing CDCA5.; Authors - Xiaoying Jin
human	IHC,WB	Int J Mol Sci; Identification of Candidate Genes in Breast Cancer Induced by Estrogen Plus Progestogens Using Bioinformatic Analysis; Authors - Yu Deng
human	WB,IHC	Cancer Cell Int; Decoding the immune microenvironment: unveiling CD8 + T cell-related biomarkers and developing a prognostic signature for personalized glioma treatment; Authors - Xiaofang Lin